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Intestinal proteome of rainbow trout is sensitive to dietary phosphorus (P) deficiency
Author(s) -
kirchner severine,
li hong,
ferraris ronaldo
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1402-c
Subject(s) - rainbow trout , proteome , trout , biology , transcriptome , intestinal mucosa , medicine , endocrinology , gene expression , chemistry , biochemistry , fish <actinopterygii> , gene , fishery
The dietary requirement for P has been estimated in fish by conventional indicators of P status, such as growth rates or bone P levels, which do not detect early stages of dietary P deficiency. We recently demonstrated P‐induced changes in the intestinal transcriptome of trout, then proved the reliability of molecular markers to acutely monitor P status. Because protein expression is more predictive of changes in function, we studied dietary P‐induced changes in the intestinal proteome of rainbow trout. Trout were fed for 20d with a Low‐P (LP) or a High‐P (HP) diet. As expected, P‐deficient fish exhibited decreased plasma and bone P contents, whereas intestinal Na:P co‐transporter gene expression and P uptakes increased. Proline and glucose transporter activity or expression were independent of diet. The most P‐responsive fish were selected for proteomic screening using previously validated molecular markers of P status. 2D gel electrophoresis of intestinal mucosa revealed that although almost all spots were expressed at seemingly similar levels, 6–10 proteins clearly exhibited a change of abundance between trout fed LP and HP diets. MALDI‐TOF mass‐spectrometry was used to sequence these potentially P‐responsive proteins from the small intestine. Intestinal proteome response to diets with varied P levels should identify reliable biomarkers of dietary P deficiency (USDA 04‐35206‐14154; 03‐35102‐13520).