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A Comparative Proteomic Analysis of Bronchoalveolar Lavage Fluid in Rats with Aging using 2‐DIGE and MALDI‐ToF/ToF
Author(s) -
Umstead Todd M.,
Freeman Willard M.,
Phelps David S.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1401-c
Subject(s) - bronchoalveolar lavage , lung , juvenile , difference gel electrophoresis , proteomics , medicine , immunology , physiology , pathology , chemistry , biology , biochemistry , genetics , gene
There are many changes associated with normal aging of the lung that can play a role in the way the lung responds to environmental insults and contribute to the increased incidence of lung disease in the elderly. This study describes the quantitative comparison of the proteomic profiles of bronchoalveolar lavage (BAL) from F344 rats of different ages, including juvenile (1mo), adult (2mo), and aged rats (18mo), using 2‐dimensional difference gel electrophoresis (2‐DIGE) for protein quantitation and with tandem mass spectrometry (MALDI‐ToF/ToF) for protein identification. We detected and compared 427 proteins, of which 130 were subsequently analyzed by MALDI‐ToF/ToF. Age group comparisons of BAL proteins showed significant changes in protein levels between juvenile and adult rats, juvenile and aged rats, and adult and aged rats for 80, 66, and 49 of the proteins, respectively. Of these changes, about twice as many increases were detected vs decreases. In each set of comparisons, about half of the significant changes were greater than 1‐fold. Differences in protein expression covered many classes and functional categories of proteins. The results reported here could provide new insight into the basis for age‐related differences in the incidence of various respiratory ailments and provide potential therapeutic targets for the treatment of lung diseases, especially those that affect the elderly.