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The Cardiac function of aged rats are more susceptible to ischemia/reperfusion injury:Role of Nitric Oxide
Author(s) -
Tian Jue,
Jia Jia,
Wu Ye,
Yang XiaoLi,
Lv XiaoPing,
Ma XinLiang,
Liu HuiRong
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1401-a
Subject(s) - nitrotyrosine , medicine , nitric oxide , reperfusion injury , ventricle , cardiac function curve , ischemia , cardiology , basal (medicine) , nitric oxide synthase , anesthesia , heart failure , insulin
Objective: To determine whether the cardiac function of aged rats is more susceptible to ischemia/reperfusion injury than that of adult heart and, if so, to clarify the mechanisms. Methods: SD rats were randomly divided into 4 groups: normal adult group (5–6 months), normal old group (22–24 months), adult‐myocardial ischemia/reperfusion (MI/R, the rats were subjected to 30 minutes of myocardial ischemia via ligating the left anterior descending coronary artery followed by 3 hours of reperfusion) group and old‐MI/R group. The cardiac function of left ventricle was continuously measured. The content of total nitric oxide (NOx) and nitrotyrosine, and the expression of iNOS in myocardium were detected. Results: A significantly exacerbated cardiac reperfusion injury was found in old‐MI/R group as evidenced by aggravated cardiac function compared with adult ‐MI/R group, especially the diastolic function. Aged hearts had a markedly increased basal NOx compared to young adult hearts (1.3 folds, P <0.05). Marked higher myocardial NOx contents, nitrotyrosine contents and iNOS expression was found in old‐MI/R group than that in adult ‐MI/R group. Conclusion: Our results demonstrated that in aged hearts high levels of NO might form iNOS attributed to the increased susceptibility of ischemic‐reperfusion injury in cardiac function.