Premium
THE IMPACT OF TEMPERATURE (37,42 and 60 0C/60 min.) ON DELTA‐AMINOLEVULINIC ACID DEHYDRATASE ACTIVITY IN BLOOD OF CHICKEN EMBRYO
Author(s) -
ELEZAJ ISA REXHEP,
SELIMI QERIM ISAK,
LETAJ KASUM RRUSTEM
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1400-c
Subject(s) - incubation , hatching , embryo , chemistry , andrology , incubation period , dehydratase , zoology , biology , biochemistry , enzyme , medicine , microbiology and biotechnology
The impact of temperature (37, 42 and 60°C/60 min.) on δ‐aminolevulinic acid dehydratase activity (ALA‐D; E.C. 4.2.1.24), were analyzed in the blood of chicken embryos from the 18 th until 21 st day of incubation and from the 1 st to the 5 th day after hatching. The ALA‐D activity was significantly higher in the hemolysate incubated in 42 and 60 °C/60 min. compared with the activity of ALA‐D incubated in 37 °C/60 min of each respective day (P<0.001). The highest ALA‐D activity (at 42 °C) was recorded on 21 st day of incubation (5803 ± 437) while the lowest activity was recorded on 5 th hatching day (4821±741). The activity of ALA‐D showed termostability on the 60 °C/60 min. in the blood of embryos (from 18 – 21 day of incubation), and in the blood of chicken from 1 st –5 th hatching day.