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The ontogeny of beta transducin repeat containing protein mRNA expression during development of the chicken limb
Author(s) -
Ladd Megan,
Everman David,
Maze Jennifer Richter
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1394-c
Subject(s) - biology , ectrodactyly , messenger rna , phenotype , limb development , gene , microbiology and biotechnology , genetics , ectodermal dysplasia
Beta transducin repeat containing protein (BTRC) is an F‐box‐WD40 protein that is involved in the NFkB signaling transduction pathway. Studies have shown that the NFkB pathway is directly involved in limb development. Ectrodactyly or split hand – split foot malformation (SHFM) is characterized by hypoplasia/aplasia of the central digits. SHFM has been observed in several species including mice, chicken and humans. In both mice and humans, it is due to a chromosomal rearrangement of the DACTYLIN gene on Chromosome 10q24. Previous studies have shown that this duplicated region contained an extra copy of DACTYLIN as well as an extra copy of BTRC. Total RNA was collected from whole chick embryos (E 0 – 7) and analyzed for BTRC expression using reverse transcription‐polymerase chain reaction (RT‐PCR). BTRC was first detected on day 1, with highest expression between E 2 – 4. This is consistent with limb development in chicken. Now that BTRC expression patterns have been determined, we will test to see if overexpression of BTRC in the chick limb bud reveals the SHFM phenotype as observed in mice and humans.