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Differential expression of P2X/P2Y genes in conventional swine tissues
Author(s) -
Hong Yonggeun,
Jeon Jaecheol,
Rho Gyujin,
Kim Jooheon
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1394-a
Subject(s) - purinergic receptor , p2y receptor , receptor , agonist , biology , ppads , medicine , endocrinology , gene expression , p2 receptor , gene , biochemistry
The aim of this study was to address the differential expression of purinergic receptor encoding gene and their bioactivity profiles in tissues of neonatal pigs. Neonatal piglets of either sex on day 1 and 5 were sacrificed. Dissected organs were used for molecular analysis of gene and protein for purinergic receptors. In addition, functional analysis of P2X/P2Y receptor by pharmacophysiological skill in swine renal artery was performed. As results, Differences exist in specific P2X/P2Y gene expression between 1 day‐ and 5 day‐old neonatal pig tissues of either sex, especially brain tissue. No significant differences of P2X/P2Y protein expression in brain, heart, kidney under with/without ATP, a P2 receptor agonist were observed. In functional analysis of P2Y receptor for vascular relaxation, 2 msATP, a strong agonist of P2Y receptor, induced relaxation of NA‐precontracted renal artery in a dose‐dependent manner. Vascular relaxative effect of ATP, 2 msATP, agonists of P2Y receptor, abolished by treatment of reactive blue 2 (RB‐2), a putative P2Y‐receptor antagonist. These findings reveal a tissue‐specific differential gene expression of purinergic receptors that were modulated by developmental stages in swine. Finally, the relationship between molecular and functional characteristic of P2X/P2Y during developmental stage in conventional pig should be applied to clinical fields. This work was supported by the Research Project on the Production of Bio‐organs (No. 200508010801), Ministry of Agriculture and Forestry, Republic of Korea.