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Oxytocin and Vasopressin heteronuclear RNAs are differentially expressed in the rat supraoptic nucleus
Author(s) -
Yue Chunmei,
Fields Raymond L,
House Shirley,
Gainer Harold
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1392
Subject(s) - supraoptic nucleus , vasopressin , oxytocin , riboprobe , in situ hybridization , endocrinology , medicine , biology , nucleus , microbiology and biotechnology , lactation , messenger rna , gene , biochemistry , genetics , pregnancy
Using intronic riboprobes and quantitative in situ hybridization (ISHH), we previously found that a riboprobe directed against intron2 of oxytocin (OT) detected larger quantities of OThnRNA in the rat supraoptic nucleus (SON) than an intron1‐directed riboprobe (Yue, et al. 2006 Am J Physiol, 209: ). We concluded that intron1 is excised from the primary OT transcript more rapidly than intron2. Here, we test this hypothesis using a two‐step, quantitative SYBR Green real‐time PCR method to measure copy numbers of OT and Vasopressin (VP) mRNA and hnRNA in adult male, female, pregnant and lactating rat SONs. Standard curves were constructed using synthetic 90bp sense‐strand oligonucleotides for quantification of copy numbers. We found consistent with our ISHH data that the OT hnRNAs containing intron2 are 1000‐fold higher than OT hnRNAs containing intron1. In contrast, VP hnRNAs containing intron1 are only 10‐fold greater than VP hnRNAs containing intron2. Measurements of OT and VP mRNAs and hnRNAs in adult male, female and 19‐day pregnant rats were similar, whereas lactation caused increases in both OT and VP hnRNAs and mRNAs, but did not alter their intron1/intron2 ratios. These data suggests that under basal and stimulated conditions, the splicing of OT intron1 is more rapid than that of intron2, whereas VP intron1 and intron2 may be removed at similar rates.