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Apoptosis of Neutrophils from Diabetic rats
Author(s) -
AlbaLoureiro Tatiana Carolina,
de Lima Thaís Martins,
Lagranha Cláudia Jaques,
Mendonça José Roberto,
PithonCuri Tânia Cristina,
Curi Rui
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1346
Subject(s) - apoptosis , streptozotocin , endocrinology , medicine , intraperitoneal injection , dna laddering , flow cytometry , dna fragmentation , diabetes mellitus , andrology , chemistry , immunology , programmed cell death , biochemistry
Several studies have shown impairment of neutrophil function that leads to high incidence of infections in diabetes. Neutrophils obtained from streptozotocin (STZ)‐induced diabetic male Wistar rats (65mg/Kg b.w, 6 weeks) were investigated. Diabetic (D) and control (C) rats received an intraperitoneal injection of glycogen solution (1%). After 4 hours, the following parameters were measured: total cell counts in peritoneal lavage; apoptosis process in neutrophils by flow cytometry; activities of caspases 3, 8 and 9 by fluorimeter and expression of the Bax, Fas, p53 and beta2M were also determined by real‐time PCR. Neutrophils from diabetic rats presented a decrease in cell viability and an increase of DNA fragmentation. Phosphatidylserine externalization was observed at the same proportion in neutrophils of C, D and treated D. The caspase 8 activity was decreased (10%) in neutrophils from D rats and these cells presented an increase of caspase 9 activity (26%), whereas the caspase 3 activity was not changed. Neutrophils from D rats presented markedly increased in Bax (9x), Fas (12x) and p53 (13x) mRNA expression. Neutrophils from D treated with insulin (2 to 4 UI/ day) during 6 weeks presented the same changes of the cells from C rat. Therefore, diabetic state induces marked changes in neutrophil apoptosis and this may be important for the impairment in neutrophil function observed under these conditions. Research support by: FAPESP and CNPq