Premium
Exercise‐Induced Muscle Damage and Apoptotic Protein Expression in Immune Cells
Author(s) -
Park KyungShin,
Sedlock Darlene A.,
Navalta James W.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1345
Subject(s) - apoptosis , analysis of variance , biology , andrology , microbiology and biotechnology , medicine , endocrinology , biochemistry
We previously reported ( Med Sci Sports Exerc 2006) a higher proportion of apoptotic lymphocytes and neutrophils 24h and 48h after a ‐10% downhill run (DH1) compared to level running (L) or a second downhill run (DH2). We also found greater muscle damage and lower antioxidant status 24h after DH1. PURPOSE: to determine if the elevation in apoptotic leukocytes was mediated by changes in Bcl‐2 and Bax protein expression by further analyzing the blood samples. METHODS: 12 moderately trained subjects (mean±SE age=22.7±0.7yr, VO 2 max=53.1±1.3ml.kg −1 .min −1 ) performed three 40 min treadmill runs at ~70% VO 2 max: L followed by DH1 and DH2. Blood samples taken at rest (PRE) and immediately, 2h, 24h, and 48h after each run were further analyzed for Bcl‐2 and Bax protein expression (ELISA). Data were analyzed using a 2‐way repeated measures ANOVA with post hoc Tukey tests. RESULTS: Bax concentration was higher in DH1 than L and DH2 (P<0.01). Bax at 24h was greater than PRE (P<0.05) following DH1. No significant changes were found for Bcl‐2. Bax/Bcl‐2 ratio was greater in DH1 than L (P<0.01) and DH2 (P<0.05). At 24h post, Bax/Bcl‐2 ratio was higher in DH1 than L and DH2 (P<0.01). In DH1, Bax/Bcl‐2 ratio at 24h (P<0.01) and 48h post (P<0.05) was elevated above PRE. CONCLUSIONS: Increased oxidative stress following muscle damaging exercise may alter the Bax/Bcl‐2 ratio in white cells towards a pro‐apoptotic state.