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N‐CADHERIN PROTECTS C2C12 MYOBLASTS FROM SERUM‐STARVATION‐INDUCED APOPTOSIS
Author(s) -
Wang Yan,
Always Stephen E.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1343
Subject(s) - c2c12 , apoptosis , cadherin , myocyte , microbiology and biotechnology , starvation , biology , chemistry , cell , endocrinology , myogenesis , biochemistry
The present study was designed to determine the role of N‐cadherin in protecting C2C12 myoblasts from apoptosis induced by serum starvation. Upon serum starvation, caspase‐9 and 3 were activated in a time‐dependent manner in C2C12 cells. Cell death index, measured by DNA fragmentation ELISA assay, was also increased in serum starved C2C12 cells. In addition, the expression level of N‐cadherin protein was elevated in response to serum starvation in a time‐dependent manner. Treatment of C2C12 cells with EGTA, which disrupts cadherin‐dependent cell contacts and signaling by depleting calcium, exaggerated apoptosis of C2C12 induced by serum starvation. Specific knock‐down of N‐cadherin protein expression by RNA interference did not induce apoptosis of C2C12 cells directly, but sensitized C2C12 cells to serum‐starvation‐induced apoptosis. Taken together, these data indicate that N‐cadherin plays a role in protecting C2C12 myoblasts from apoptosis induced by serum starvation. Supported by NIH AG021530.