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Distal lung fluid absorption is induced in preterm guinea pigs with low tidal volume ventilation
Author(s) -
Koshy Shyny,
Li Tianbo,
Folkesson Hans G.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1340-b
Subject(s) - medicine , ventilation (architecture) , lung , ards , tidal volume , respiratory distress , anesthesia , fetus , continuous positive airway pressure , respiratory system , pregnancy , biology , mechanical engineering , obstructive sleep apnea , engineering , genetics
Low tidal volume ( TV ) ventilation is clinically useful in acute respiratory distress syndrome ( ARDS ) patients. Traditionally, ARDS patients have been ventilated with TVs to maintain blood oxygenation and prevent hyperkapnia. Our objective was to determine if low TV ventilation would be beneficial for distal lung fluid absorption in preterm guinea pigs at risk of respiratory distress syndrome ( RDS ). We ventilated preterm guinea pigs at 64–66 days gestation age with low TVs ( 6 ml/kg ) and compared with the more traditional TV ( 12 ml/kg ). Guinea pig fetuses oxygenated by CPAP ( continuous positive airway pressure ) served as reference points. Distal lung fluid absorption was measured after intratracheal instillation of 5% albumin in 0.9% NaCl by mass‐balance. Low TV ventilation resulted in a 23 ± 10% distal lung fluid absorption, which compared to −2 ± 6% in CPAP oxygenated fetuses and −1 ± 3% in high TV ventilated fetuses. αENaC and CFTR mRNA expression was measured by RT‐PCR. The increased distal lung fluid absorption in low TV ventilated fetuses was associated with an increase in αENaC mRNA. CFTR mRNA remained the same in all three groups. Thus our data suggest that low TV ventilation increases fetal distal lung fluid absorption by an ENaC‐dependent mechanism and thus may be preferential to use clinically over high TV ventilation. ( Funded by March of Dimes Birth Defects Foundation Research Grant No. 6‐FY03‐64 )