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Sry increases sodium reabsorption in the Wistar Kyoto rat kidney
Author(s) -
Hart Michael,
Salisbury Ron,
Milsted Amy,
Turner Monte,
Ely Daniel
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1331-b
Subject(s) - testis determining factor , medicine , endocrinology , natriuresis , excretion , chemistry , renal function , creatinine , reabsorption , kidney , urine , y chromosome , biology , gene , biochemistry
Our lab has identified a locus on the Y chromosome of the Spontaneously Hypertensive Rat that is associated with a 20–25 mmHg increase in Blood Pressure (BP). A candidate for this locus is the Sry gene, also known as the testis determining factor. We have shown that Sry inserted into a mammalian expression vector and electroporated into the kidney of the normotensive Wistar‐Kyoto rat, produced a 50% increase in plasma norepinephrine content after 2 weeks compared to vector controls. The aim of the current research was to investigate if exogenous Sry increased the activity of the renal Na + /K + ATPase pump and decreased urinary Na + excretion in the WKY strain. Age‐matched WKY adult male rats were electroporated either with the Sry expression construct or an empty vector and kidney function evaluated by 24 hr creatinine clearance, urine Na + excretion rate, urine electrolyte concentration by flame photometry and BP by tail‐cuff sphygmomanometry. At 11 days post‐electroporation no difference was found in GFR. However, the Sry group had an average increase in BP of 17 mmHg compared to empty vector controls (p<0.05) and excreted 33% less Na + compared to controls (p<0.05). Despite an increase in BP which increases pressure natriuresis, the Sry group retained more Na + than the empty vector controls. Our results are consistent with the idea that one function of Sry may be renal regulation of Na.