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Primary cultures of enterocytes exhibit non‐genomic adaptive regulation of carrier‐mediated glucose absorption
Author(s) -
Kimura Yasuhiro,
Braasch Dwaine A,
Turner Jerrold R,
Buddington Randal K
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1327-b
Subject(s) - enterocyte , adenosine , caco 2 , cell culture , microbiology and biotechnology , small intestine , ileum , biology , glucose transporter , chemistry , endocrinology , medicine , biochemistry , genetics , insulin
Exposure of mouse small intestine to adenosine and AMP (5 mM) rapidly (<10 min) and reversibly increases (~2‐fold) glucose uptake. Since the associated signaling networks and mechanisms of the responses are difficult to define using intact tissues, we sought a cell based approach. Although the colon adenoma Caco‐2 cell line is commonly used as an enterocyte model, exposure to adenosine and AMP decreases glucose uptake by 70% and 15%, respectively. IEC‐6 cells, an undifferentiated cell line derived from rat ileum, respond to adenosine and AMP by a 3‐fold increase in glucose uptake. However, cancerous cells of a different tissue origin or undifferentiated cells that require stimuli to induce differentiation raise concerns of relevance. Therefore, we evaluated primary cultures of enterocytes harvested from BALB/c mouse small intestine that were cultured on PET membrane inserts for 2‐ 24 h. Accumulation of 4 μM glucose after a 10 min exposure to adenosine and AMP (25 mM) peaked after 8 h of culture and was 6‐ and 9‐fold higher relative to controls. We contend primary cultures of freshly harvested enterocytes provide data that are physiologically relevant, do not require long‐term, rigorous culture conditions, and can be used to explore the signaling networks and mechanisms responsible for non‐genomic responses.