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Correlation between lean skin water and lean body water during acute dehydration
Author(s) -
Campbell Shan E.,
Chung Peggy A.,
Ostrowski Rafael,
Hoarau Carine,
Debreczeny Martin P.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1312-a
Subject(s) - body water , dehydration , dilution , chemistry , water body , zoology , extracellular fluid , volume (thermodynamics) , body fluid , lean body mass , body weight , medicine , environmental science , biology , extracellular , biochemistry , environmental engineering , physics , quantum mechanics , thermodynamics
To determine the relationship between total body water (TBW) and local water content measured in the skin (SW) this study assessed 8 piglets in a dehydration model. Total body water was assessed by deuterium oxide (D2O) dilution throughout the experiments, and by whole body carcass analysis at the end of each experiment. Additionally extracellular water and plasma volume were assessed using bromide dilution and Evan’s blue, respectively. Skin water was assessed by tissue biopsies, taken at 60 min intervals throughout the experiment. Lean body water (LBW) and lean skin water (LSW) were assessed by extracting the fat from the carcass and biopsy samples. A correlation does exist between TBW and SW with r2=0.558 (P<0.05), however, the strongest correlation occurred between LBW and LSW with r2=0.844 (P<0.05) and a standard error of prediction of 0.87%. These data demonstrate that LSW gives an accurate and precise estimate of LBW, and could therefore be used to determine the hydration index in appropriate research settings.