Premium
P2Y receptor modulation of XII inspiratory motor output in neonatal rat
Author(s) -
de Souza Alvares Tucaaue,
Huxtable Adrianne,
Ireland Matthew,
Adachi Tadafumi,
Poon Betty,
Lorier Amanda,
Funk Gregory
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1295-d
Subject(s) - tonic (physiology) , receptor , agonist , chemistry , medicine , neuroscience , endocrinology , biophysics , biology
Despite a focus on P2X receptors (Rs), new data have shown that inspiratory rhythm generating networks in the Pre‐Bötzinger complex are very sensitive to P2Y 1 R modulation. Here we used nerve and whole‐cell recording in rhythmic and non‐rhythmic medullary slices of neonatal rat to assess the effect of P2YR signaling on XII inspiratory activity and motoneuron (MN) properties respectively. Local application of UTP (1‐10 mM; agonist at P2Y 2,4,6,11–14 ) to the XII nucleus evoked tonic discharge in XII nerve and a small but significant increase (<20%) in burst amplitude. UTP (1 mM) also evoked slow inward currents (150±30pA, n=11) that reversed near E K+ , and increased input resistance by 15±5%. Local application of P2Y 1 R agonists 2MeSADP (1‐10mM) and MRS2365 (0.1‐1mM) to the XII nucleus also evoked tonic discharge but a 30–50% increase in burst amplitude. P2Y 1 R involvement was further supported by P2Y1R immunolabeling and slow onset, small amplitude 2MeSADP‐evoked (1‐10mM) inward currents (43±21 pA, n=6) that reversed near E K+ (3/6 MNs). Data indicate functional P2YRs on XII MNs. Despite UTP currents that were 4‐fold greater than P2Y 1 R currents, P2Y 1 R agonists appear to have had larger effects on XII inspiratory burst amplitude. P2Y 1 R may be preferentially distributed to inspiratory XII MNs. Funded: CIHR, AHFMR, CFI, MFN