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Reactive oxygen species production during chronic ventilatory hypoxia
Author(s) -
Hodyc Daniel,
Johnson Eric,
Hnilicková Olga,
Smolková Pavlína,
Herget Jan
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1287
Subject(s) - hypoxia (environmental) , superoxide , chemistry , oxygene , endocrinology , medicine , reactive oxygen species , oxygen , biochemistry , enzyme , organic chemistry
Pulmonary hypertension resulting from chronic hypoxia is at least partly caused by increased production of reactive oxygen species. Wistar rats were kept for 1, 4 and 21 days in isobaric hypoxic chamber (FiO2 = 0,1), while controls stayed in normoxia. Using chemoluminiscence analyser, we compared NO production in expired air, plasma and perfusate drained from isolated rat lungs immediately after their removing from hypoxia. Using spectrophotometrical analysis of the perfusate drained from isolated lungs we also measured superoxide concentration. Reduction of cytochrome c in the presence of SOD was subtracted from the values without SOD. In expired air we found the highest NO production after 1 and 4 days in hypoxia (H1: 699,9, H4: 656,6, H21: 412,4, N: 265,7 [pg.min‐1.100g‐1], p<0,001). The plasmatic concentration of NO oxidative products (NO2‐ and NO3‐) was significantly increased after 1, 4 and 21 days in chronic hypoxia compared to controls. (H1: 29,8, H4: 37,1, H21: 28,4, N: 20,0 [ìM], p<0,05) In the perfusate we found significantly highest NO production after 1 day (H1: 5,1, H4: 2,7, H21: 1,5, N: −0,14 [ìM. g‐1 ], p<0, 01), whereas superoxide concentration started to increase after 4 days in hypoxia (H1: −0,06, H4: 2,64, H21: 0,54, N: 0,94 [ìM. g−1], p<0,05). In rats exposed to chronic hypoxia the onset of increased NO production precedes the increase in superoxide production. Supported by GAUK 2005/45/C, MSMT 1M 0510.