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Aldosterone Upregulates the Brain Renin‐Angiotensin System in Rats with Heart Failure
Author(s) -
Yu Yang,
Wei ShunGuang,
Zhang ZhiHua,
GomezSanchez Elise P,
Weiss Robert M,
Felder Robert B
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1266-b
Subject(s) - medicine , endocrinology , aldosterone , hypothalamus , renin–angiotensin system , angiotensin ii receptor type 1 , angiotensin ii , heart failure , mineralocorticoid receptor , chemistry , receptor , blood pressure
Activation of the brain renin‐angiotensin system (RAS) contributes to increased sympathetic drive in heart failure (HF). Plasma aldosterone (ALDO), a downstream product of the systemic RAS, crosses the blood‐brain barrier. In peripheral tissues, ALDO enhances the action of the RAS. We hypothesized that ALDO increases in the brain of HF rats and upregulates brain RAS activity. Sprague Dawley rats underwent coronary ligation to induce HF or sham surgery (SHAM). Compared with SHAM (n=5), HF (n=5) rats had high (∗P<0.05, HF vs SHAM) ALDO levels (by ELISA) in plasma (288±32∗ vs 188±25 pg/ml) and in hypothalamus (240±21∗ vs 165±19 pg/g tissue) but not in cortex. Some HF rats were treated for 4 weeks with intracerebroventricular (ICV) RU28318 (1 μg/hr, n=9), a selective mineralocorticoid receptor antagonist, or vehicle (VEH, n=10). Compared with untreated SHAM (n=9), HF+VEH rats had increased (¥P<0.05) mRNA expression for angiotensin converting enzyme (ACE, 0.031±0.003¥ vs 0.013±0.002) and angiotensin type 1 receptor (AT1‐R, 0.021±0.002¥ vs 0.009±0.001) in hypothalamus by real‐time PCR (normalized by GAPDH), and more excited (Fra‐LI positive) neurons (65±5¥ vs 29±6) in paraventricular nucleus (PVN) of hypothalamus by immunohistochemistry. Compared with HF+VEH rats, HF+RU28318 rats had less (#P<0.05) hypothalamic ACE (−29%#) and AT1R mRNA (−34%#) and fewer Fra‐LI positive neurons (−27%#) in the PVN. No effects on these variables were found in brain cortex. RU28318 had no effect on plasma ALDO. Together, these two sets of data suggest that an increase in hypothalamic ALDO in HF upregulates brain RAS activity, likely contributing to augmented sympathetic drive. Supported by NIH HL‐063915.