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Myocardial infarct size estimation by echocardiography in an acute rat model
Author(s) -
Kilvington Jason Alan,
Coleman Holly,
Hansen Beverly,
Dierks Heidi,
Zhang Weiguo
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1261-a
Subject(s) - medicine , cardiology , ventricle , myocardial infarction , ejection fraction , end systolic volume , end diastolic volume , isoflurane , infarction , diastole , stroke volume , heart failure , blood pressure , anesthesia
It has been a challenge to estimate the infarct size in rats post acute myocardial infarction (MI). The major purpose of our study was measure structural and functional changes of the left ventricle with non‐invasive echocardiography and analyze the changes based on infarct size determined by postmortem pathology.Methods: MI was induced by coronary artery ligation via left thoractomy in male Lewis rats under isoflurane. The Left ventricular end diastolic and systolic volume (LVEDV and LVESV), ejection fraction (EF) and fractional shortening (FS) were measured before surgery (for baseline) and ~24 hrs post surgery with echocardiography (VisualSonics, Vevo 770). The heart was sectioned and stained with TTC to calculated MI size (Image J Planimetry software, NIH). Results: In rats with MI >35% (57±22%, mean±SD, n=11) LVESV increased by 77±19μl (45±23 to 121±23); whereas in rats with MI <35% (12±12%, n=15) it increased by only 36±34 μl (43±22 to 79±25) (p<0.01). Correspondingly, in rats with MI >35% EF decreased by 38±17% vs. 15±12% in rats with MI <35% (p<0.01). In the same order, FS decreased by 29±8% vs. 13±11% (p<0.01). However, no significant change in LVEDV was observed (p>0.05). Conclusions: Our data indicates that the changes in LVESV, EF and FS are determined by the size of MI. By measuring these changes with echocardiography, it is possible to identify rats with a larger MI within 24 hrs.