Premium
Novel technique for estimation of hemodynamic status of rats during hemorrhage and resuscitation
Author(s) -
Callahan Michael F,
Kislukhin Victor K,
Thuramalla Naveen V,
Krivitski Nikolai M,
Smith Thomas L
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1259
Subject(s) - medicine , blood volume , resuscitation , intravascular volume status , anesthesia , jugular vein , cardiac output , hemodynamics , saline , stroke volume , femoral artery , blood flow , volume (thermodynamics) , isotonic saline , blood pressure , cardiology , heart rate , physics , quantum mechanics
There is no simple technique to assess the fluid status in rats during hemorrhage and resuscitation. This study tested the ability of ultrasound velocity dilution to monitor absolute volume changes in rats. The blood loss model (5 male Sprague‐Dawley rats) was performed in four steps: 7‐ml/kg body‐weight was withdrawn per step. All the blood was re‐infused in one step, after 40 min. Blood was circulated (8 ml/min) through an extracorporeal AV tubing loop (priming volume 1 ml) that was connected between the carotid artery and the jugular vein in the anesthetized rat. Two flow‐dilution sensors were clamped on the arterial and venous sides of the loop. Isotonic saline (0.2‐0.5 ml) was injected to measure: cardiac output (CO), central blood volume (CBV, volume in lungs and heart) and actively circulating volume (ACV, the blood volume in which the indicator mixes during one minute). A Millar 1.0F pressure (MAP) catheter was placed into the femoral artery.Ultrasound dilution was proved to be an easy tool to investigate the volume status of rats. The data suggests that CBV and ACV are the earliest predictors of blood loss.