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Plasma cytokine and muscle/leukocyte cytokine mRNA changes after cycling are not countered by quercetin
Author(s) -
Nieman David Christopher,
Henson Dru A.,
Davis J. Mark,
Murphy E. Angela,
Jenkins David P.,
Gross Sarah J.,
Carmichael Martin D.,
Quindry John C.,
Dumke Charles L.,
Utter Alan C.,
McAnulty Steven R.,
McAnulty Lisa S.,
Triplett N. Travis,
Mayer Eugene P.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1249
Subject(s) - cytokine , medicine , endocrinology , messenger rna , tumor necrosis factor alpha , interleukin 6 , skeletal muscle , placebo , interleukin , immunology , chemistry , pathology , biochemistry , gene , alternative medicine
This study investigated the effects of quercetin (Q) on exercise‐induced changes in plasma cytokines, cytokine mRNA in leukocytes and muscle, and muscle nuclear factor kappa B (NFκB). Trained male cyclists (N=40) received 3 wks Q (1000 mg/day) or placebo (P) prior to and during a 3‐d period in which subjects cycled for 3 h/day at ~57% Watts max . Blood samples were collected before and after the 3 sessions and assayed for plasma levels of IL‐6, IL‐10, IL‐1ra, IL‐8, TNF‐α, G‐CSF, MCP‐1, MIP‐1β, and MIF, and leukocyte mRNA for IL‐10, IL‐8, and IL‐1ra. Muscle biopsies were obtained before and after the first and third sessions and assayed for NFκB and mRNA expression of IL‐6, IL‐8, TNF‐α, and IL‐1β. Post‐exercise increases for all 9 cytokines did not differ significantly between Q and P groups. Cytokine mRNA expression increased significantly post‐exercise for IL‐8 and IL‐1ra in blood leukocytes and for IL‐6, TNF‐α, IL‐8 and IL‐1β in muscle, and except for leukocyte IL‐8 mRNA, no difference between groups was measured. Muscle NFκB did not increase post‐exercise and did not differ between Q and P. In conclusion, Q vs. P ingestion did not alter exercise‐induced increases in plasma levels of 9 cytokines or mRNA expression of specific cytokines measured in leukocyte or muscle. Partially supported by a grant from the Defense Advanced Research Projects Agency (DARPA) and the Army Research Office (ARO), award number W911NF‐06‐0014.