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Exercise‐Stimulated Blood Flow in Older and Younger Men: A Methodological Comparison
Author(s) -
Casperson Sha,
Dillon Edgar,
Hickner Robert,
Newberry Nelson,
Lakshman Kishore,
Mujeeb Mehrukh,
Angel James,
Urban Randal,
Chinkes David,
SheffieldMoore Melinda
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1237
Subject(s) - medicine , blood flow , sarcopenia , skeletal muscle , cardiology , femoral artery , hemodynamics , microdialysis , laser doppler velocimetry , endocrinology , central nervous system
Chronic reductions in peripheral blood flow and perhaps altered nutritive flow may contribute to sarcopenia. We compared exercise‐induced changes in blood flow between older and younger men using both macro‐ and microvascular flow techniques. Older (n=7) and younger (n=7) men were studied at rest and during recovery from moderate walking (45 min at ≈ 40%VO2peak). Whole‐leg flow was measured using ICG dye‐dilution. Femoral artery flow was determined by 2‐D Doppler ultrasound. Interstitial skeletal muscle nutritive exchange was determined via the microdialysis ethanol technique (MET). Skeletal muscle microvascular flow and recruitment was determined using contrast‐enhanced ultrasound (CEU). ICG, Doppler and MET showed significant exercise‐induced increases in both groups in whole‐leg flow, femoral artery flow and nutritive exchange, respectively (P<0.01). ICG and Doppler detected no differences between groups. MET measured no between group differences from rest through 60 min of recovery; however, by 120 min, nutritive exchange was significantly lower in the older men as compared to the younger (P<0.05). CEU measured significant increases in microvascular recruitment in both groups, but only the older men had significant increases in microvascular flow (P<0.05). These data highlight the importance of measuring microvascular flow in studies of aging and in response to a given stimuli. Funded by NIH/NIA R01 AG21539 to M. Sheffield‐Moore. Studies conducted on the GCRC at UTMB funded by grant M01 RR 00073 from the National Center for Research Resources, NIH, USPHS.