Premium
Measurement of Microvascular Oxygen Saturation (mSO 2 ) and hemoglobin concentration (m[Hb]) during Prolonged Hemorrhagic Shock (HS)
Author(s) -
Torres Lucia,
Filho Ivo P Torres,
Pittman Roland N
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1235
Subject(s) - microcirculation , medicine , mean arterial pressure , oxygenation , anesthesia , hemoglobin , arterial blood , shock (circulatory) , intravital microscopy , blood pressure , perfusion , acidosis , cardiology , heart rate
Since monitoring of microvascular and systemic parameters could be valuable for the best clinical assessing, we developed a model to assess the effects of HH on microvascular, biochemical and systemic parameters. Rats were cannulated for anesthesia, bleeding and mean arterial pressure (MAP) monitoring. The spinotrapezius muscle was exposed for intravital microscopic. During baseline, systemic variables such as blood gases, Since monitoring of microvascular and systemic parameters could be valuable for the best clinical assessing, we developed a model to assess the effects of HH on microvascular, biochemical and systemic parameters. Rats were cannulated for anesthesia, bleeding and mean arterial pressure (MAP) monitoring. The spinotrapezius muscle was exposed for intravital microscopic. During baseline, systemic variables such as blood gases, O 2 extraction ratio (O 2 ER) and hemoglobin were measured. Diameter, mSO 2 and m[Hb] of arterioles and venules were also evaluated. Rats were bled during 15–20 minute period until MAP reached 40mmHg. Hemorrhagic shock measurements were made when MAP reached 40 mmHg and at 30, 60, 120 and 180 min thereafter. Additional bleeding or Ringer’s lactate infusion were used to maintain hypotension. During HS, animals had a metabolic acidosis and hyperventilation. At 120 min of HS, mSO 2 was significantly lower than the baseline. Microvascular SO 2 showed correlation with systemic O 2 ER (r = 0.62). Microvascular [Hb] correlated with systemic hemoglobin (r = 0.94) and with mSO 2 (r = 0.63). Microvascular associated to systemic oxygen measurement may enable us to monitor changes in oxygenation tissue during HS accurately and to decide a better treatment for improving systemic and microvascular outcomes. Support: NIH HL079087.