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MECHANISM INVOLVED IN THE ETHANOL‐INDUCED CONTRACTION OF DOG PULMONARY ARTERY
Author(s) -
Jakupaj Muharrem,
Neziri Burim,
Belegu Mazllum,
Devaja Shqipe,
Krasniqi Shaip
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1231-c
Subject(s) - chemistry , contraction (grammar) , ethanol , incubation , saline , nifedipine , medicine , endocrinology , biochemistry , calcium , organic chemistry
In this study are present the results of ethanol effects on dog’s PASM (Pulmonary artery smooth muscle). We also test the role of NO and other mechanisms involved in this process. Our previously results showed that ethanol used in concentration of 46, 92,138 mM always provoke contraction of PASM and are depend to the dose. In condition when medium lacked Ca++, PASM response identically as in medium when Ca was present (27 strips form 7 dog’s).We didn’t see any effect of ethanol in constrictive response of dog’s PASM priory incubation of this tissue in different concentration of nifedipine (10,50,100 μM), as Ca++ blocker channel. In this study we test also the role of dog’s PA endothelium in modulation of response to ethanol and role of NOS and endogen NO which has released form effect of ethanol and spasmogen substances. Blockade of NOS with LNAME (7 strips from 7 dog’s) significantly increase response of PASM of the dog’s to ethanol. Values of cGMP on the tissue of dog’s PASM after incubation in medium with ethanol 322 mM are 38% lower than control tissue that was incubate in saline. These results show that ethanol provoke contraction of dog’s PASM and is dose dependent. In this response intra or extra cellular Ca++ doesn’t have any role. Endogen NO is involved in response of contraction of dog’s PASM. Generation of cGMP is inhibiting due to priory effect of ethanol.