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Desflurane induces early and delayed anesthetic preconditioning in the in vivo model of acute myocardial infarction in mice
Author(s) -
Redel Andreas,
Lange Markus,
Smul Thorsten M.,
Jazbutyte Virginija,
Roewer Norbert,
Kehl Franz
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1223-b
Early and delayed anesthetic preconditioning (APC) were demonstrated in various species 1,2 . The investigation of APC in gene‐targeted animals might provide further insights into underlying mechanisms. It is unclear if early and delayed preconditioning are induced by volatile anesthetics in mice. All procedures conformed to the FASEB Statement of Principles for the use of animals in research and education. Pentobarbital‐anesthetized BL6 mice (20–25g) were ventilated and instrumented for measurement of hemodynamics (HR, MAP). Animals received 45 min coronary artery occlusion (CAO) and 180 min reperfusion. One MAC Desflurane (15min) was administered 15min, 12h, 24h, 48h or 96h prior to CAO. Area at risk (AAR) and infarct size (IS) were assessed by Evans Blue and TTC staining, respectively. Statistics: ANOVA, posthoc Duncan; data are mean±SEM. IS was 48±6% (n=8) in control animals. IS was significantly (∗P<0,05) reduced by desflurane when administered 15min (9±2%∗, n=7) and 48h (14±3%∗, n=6), but not 12h (42±8%, n=6), 24h (36±7%, n=6) and 96h (37±11%, n=7) prior to CAO. Early and delayed APC were demonstrated for the first time in the in vivo model of acute myocardial infarction in mice.