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Neuropeptide Y (NPY) Y2 Receptor (Y2R) Mediates Dilation in Peripheral Collateral Vessels
Author(s) -
Taylor Jessica C,
Laughlin M Harold,
Terjung Ronald L
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1213-a
Subject(s) - neuropeptide y receptor , vasodilation , medicine , endocrinology , vasoconstriction , collateral circulation , acetylcholine , cardiology , receptor , chemistry , neuropeptide
Increases in shear stress and decreased transluminal pressure produce aberrant responses to vasomotor stimuli and changes in Ca 2+ ‐free vascular compliance (VC) in collateral vessels. The purpose of this study was to examine responses to NPY, NPY 13–36 (NPY fragment specific for Y2R), and acetylcholine (ACh) and alterations in VC in collateral vessels. Male SD rats (n=13) received unilateral femoral ligation {occluded/non‐occluded (OCC/NOR)} or no treatment (CON) 14 days prior to sacrifice. Arteries were dissected, cannulated, pressurized to 45 cmH 2 O and visualized via video microscopy. Dose‐responses to ACh (10 −9 to 10 −4 ), NPY 13–36 and NPY (both 10 −9 to 10 −6 ) and pressure/diameter curves (P/D) (10 to 120 cmH 2 O, Ca 2+ ‐free) were studied. OCC dilated (peak = 40%, p<0.05) in response to NPY 13–36 whereas NOR and CON did not dilate. P/D were increased from 20 to 70 cmH 2 O (p ≤ 0.02) in OCC versus NOR and CON reflecting structural remodeling following occlusion. NPY constrictions were similar among CON, NOR, and OCC. OCC showed an enhanced response to ACh versus NOR and CON. Increased Y2R contributes to the increased diameter of collateral arteries since Y2R was previously shown to be angiogenic and upregulated in these vessels. Vasodilation to NPY 13–36 in OCC indicates a functional role of the Y2R in collateral vessels. Support: HL37387, T32 AR48523