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Interaction of Resistance Exercise and BCAA Supplementation on Akt and p70 s6 kinase Phosphorylation in Human Skeletal Muscle
Author(s) -
Gallagher Philip,
Richmond Scott,
Dudley Kelli,
Prewitt Michael,
Gandy Nicole,
Kudrna Becky,
Touchberry Chad
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1206
Subject(s) - protein kinase b , skeletal muscle , phosphorylation , p70 s6 kinase 1 , ingestion , placebo , endocrinology , medicine , pi3k/akt/mtor pathway , resistance training , chemistry , biochemistry , signal transduction , pathology , alternative medicine
The purpose of this study was to examine the interaction of amino acid supplementation and resistance exercise on Akt and p70s6k phosphorylation in skeletal muscle. Four groups of healthy college‐aged males (N=5/group) volunteered for this investigation: Branched‐chain amino acid with resistance exercise (AA+EX), Branched‐chain amino acid only (AA), Placebo with resistance exercise (PCBO+EX), and Placebo only (PCBO). The diet and activity level of the subjects was controlled for 24 hours prior to each trial. Three muscle biopsies (pre (T1), and 30 minutes (T2) and 4 hours (T3) post ingestion of supplementation) were obtained during each trial. The samples were analyzed for Akt and p70s6k phosphorylation using western immunoblotting. All groups except PCBO had an increase in Akt and p70s6k phosphorylation at both T1 and T2; with AA+EX having the greatest increase (Table 1). These data suggest that resistance exercise in combination with branched‐chain amino acid ingestion induces hypertrophic signaling in skeletal muscle. Research supported, in part, by a University of Kansas New Faculty General Research Fund grant (P. Gallagher). 1 Phosphorylation levels of Akt and p70s6k in human skeletal muscle