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Studies on oxyradical, lipid peroxidation, cytochrome C and mitochondrial calcium in myocardium from diabetic rats
Author(s) -
Wang ShuQiu,
Kang YuMing,
Qin WenBo,
Lu ChunFeng,
Wang ShuXiang,
Zhang ShiLing,
Chen TingYu,
Ma XiaoRu,
Liang YanFeng,
Wang FangFang,
He RongLi
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1198
Subject(s) - endocrinology , medicine , malondialdehyde , lipid peroxidation , calcium , superoxide dismutase , mitochondrion , chemistry , nitric oxide synthase , cytochrome c , nitric oxide , oxidative stress , biology , biochemistry
The present study was undertaken to observe the difference of nitrogen monoxidum (NO), malondialdehyde (MDA), cytochrome C (Cyt‐C), nitric oxide synthase (NOS), mitochondrial calcium and superoxide dismutase (SOD) in myocardium between noninsulin dependent diabetes mellitus (NIDDM) rats and normal rats. Methods: Twenty NIDDM rats were made, and 20 normal rats were used as control. The levels of NO, MDA, Cyt‐C, NOS, mitochondrial calcium and SOD in myocardial cells from all rats were measured, and histological changes were observed. Results: Compared with control, the levels of NO (2.28±0.40∗ vs 1.81±0.33 μmol/g protein), NOS (0.49±0.09∗ vs 0.39±0.07 U/mg protein), MDA (7.87±0.31∗ vs 7.36±0.53 nmol/mg protein), cytoplasma Cyt‐C (3.68±0.17∗ vs 3.25±0.34 g/L) and mitochondrial calcium (2.90±0.35∗ vs 2.47±0.23 μg/L) were higher (NIDDM rats vs control, ∗P<0.05) in the NIDDM rats. But, the levels of mitochondrial Cyt‐C (0.19±0.05∗ vs 0.28±0.06 mmol/L) and mitochondrial SOD (0.81±0.21∗ vs 1.18±0.39 U/mg protein) were lower (NIDDM rats vs control, ∗P<0.05) in the NIDDM rats compared with control. Under microscope, myocardium deliquescenced and mitochondria swelled, and inflammatory cell infiltrated in mesenchyma. Conclusions: Oxyradical, lipid peroxidation, Cyt‐C and mitochondrial calcium are involved in the damage of myocardium in NIDDM rats.