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Fructose during suckling and into weaning promotes increased body weight in young adult rats prior to affecting glucose or fatty acid metabolism
Author(s) -
Bomhof Marc Richard,
Wright David C,
Hoedl Abha,
Turchinsky Joan,
Coumans Will,
Luiken Joost JJP,
Bell Rhonda C
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1197-a
This study examined the effect of initiating fructose (FR) feeding during suckling and maintaining FR post‐weaning on body weight, glucose and fatty acid metabolism. Rat pups (12 days old) were randomly assigned to 1 of 3 diets; suckle controls (SC), FR‐containing formula, or rat milk substitute (RMS) formula. FR or RMS formulae were given by artificial rearing until 20 days old. From weaning until 6 weeks of age, half of SC were fed AIN 93G (SC‐Chow) while all others were fed a high FR diet. At 6 wks, FR rats were heavier than RMS and SC‐FR, though not SC‐Chow (FR=185±13; RMS=173±10;SC =160±12;SC‐Chow =183±2). Retro‐peritoneal fat pads were heavier in FR vs. SC‐FR (2.16±0.24 vs. 1.00±0.22 g, p<0.013), but did not differ among other groups. Oral glucose tolerance was similar in all groups but FR rats were moderately hyperinsulinemic following the glucose challenge (p<0.12). In muscle, GLUT4 content was reduced by ~30% in FR rats vs. all other groups (p<0.05) although PGC‐1 protein mass was unaffected by diet. Fatty acid uptake into membrane vesicles from heart, muscle and adipose did not differ among groups, and SREBP‐1c content in adipose tissue was not altered by diet. Continuing exposure to dietary FR promotes increased body weight and adipose accumulation, possibly through changes in insulin but not SREBP‐1c and PGC‐1. Impairment in glucose tolerance and fatty acid uptake may emerge as rats mature. Funded by NSERC and AHFMR.