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Renal Function Alterations in Ex‐Vivo Perfusion in Diabetic Rats
Author(s) -
Fonteles Manassés Claudino,
Longo Vitoria M,
Farah Vera,
Fiorino Patricia,
Irigoyen MariaClaudia
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1195
Subject(s) - streptozotocin , renal function , perfusion , medicine , diabetic nephropathy , endocrinology , diabetes mellitus , kidney , renal blood flow , urology , in vivo , chemistry , biology , microbiology and biotechnology
Diabetic nephropathy is a major complication associated with poor diabetic control and a leading cause of end stage renal failure. We have developed a model to study early events of this pathology by using Wistar male control (Ct, n=6) and diabetic rats (n=5) induced by streptozotocin (STZ, 50mg/kg, iv, 21 days). The animals were anaesthetized with sodium pentobarbital (50 mg/kg), the right kidney was removed without interruption of the flow and placed in the perfusion system containing Krebs‐Henseleit solution plus 6% bovine serum albumin. Inulin clearance (GFR), renal vascular resistance (RVR), sodium tubular transport (%TNa+), potassium tubular transport (%TK+) and chloride tubular transport (%TCl ¯ ) were determined every 10 min. In STZ group we observed an increase in RVR, with peak values at 60 min perfusion time (STZ: 28±9 vs Ct: 5±0.03 mmHg/mL/g/min), associated with an extremely reduced in GFR (STZ: 0.2±0.04 vs Ct: 0,8±0.06 mL/g/min). The TNa+ (STZ: 33±2 vs Ct: 88±1 %), TK+ (STZ: 33±7 vs Ct: 67±2 %), and TCl ¯ (STZ: 34±7 vs Ct: 86±0.1 %), were also reduced in STZ rats. Conclusion: These preliminary findings demonstrate that a major defect has been observed in STZ kidneys that is reflected on RVR, GFR and ionic dynamics measured during renal perfusion. This model could be a very important tool in order to evaluate early changes observed in experimental diabetes.