Effect of the Ointment Added with Carbonized Pine Cone on Wound Healing of Burn Injuries Using Laser Irradiation in Guinea Pigs

This study was conducted to determine whether treatment of 5 ointments added with carbonized pine cone along with the control might have notable effect on the cell regeneration following burn injuries. Wounds were made by long‐pulsed Er:Yag laser on the back of 16 guinea pigs. Laser was delivered with 8 passes on each wound. The laser‐irradiated lesions were photographed by digital camera. Skin samples were fixed for H & E stain, Masson’s trichrome stain, and elastic fiber stain or immunohistochemical analysis of collagen I, III, IV, keratin 6(K6), keratin 10(K10), and involucrin. Dermatologist evaluated all sides. In findings, ointment 1 seemed to be the best in wound healing at day 14. On microscopic examination, newly formed collagen fibers and capillaries were denser in dermal areas with ointment 1, 2 and 3. Elastic fibers were more prominent in ointment 1 applied area. In epidermal formation, K6 was moderately manifested at week 2 with the ointment 1 and 3, and the control. But, K6 manifestation was decreased in the area with ointment 1 and 3, and increased in the area of the control at week 3. While K10 strongly appeared in the area with ointment 1. Involucrin also appeared the strongest in the areas with ointment 1, 2, and 3. In conclusion, the ointments added with pine cone seemed to be effective in wound healing and the best among the treatments was ointment 1.