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DNA microarray analysis suggests a regulatory role of human estrogen sulfotransferase SULT1E1 in the expression of signal transduction‐related genes in human prostate cancer CA‐HPV‐10 cells
Author(s) -
Kapoor Ruchita,
Sheng Jonathan
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1186-b
Subject(s) - biology , signal transduction , transfection , estrogen , estrogen receptor , microarray analysis techniques , gene expression , sulfotransferase , regulation of gene expression , cancer research , microbiology and biotechnology , gene , cancer , endocrinology , genetics , sulfation , breast cancer
Human estrogen sulfotransferase catalyzes the sulfation of estrogen at the 3‐hydroxyl position. Studying the roles of estrogen sulfotransferase in the regulation of estrogenic action in human cells could provide valuable information for the treatment and prevention of human estrogen‐associated diseases such as prostate cancer. The main purpose of this work is to elucidate the regulatory role of human estrogen sulfotransferase SULT1E1 in the signal transduction‐related gene expression in human prostate cancer CA‐HPV‐10 cells. Expression of intracellular signal transduction‐related genes in CA‐HPV‐10 cells following SULT1E1 transfection was investigated by DNA microarray analysis. Notable changes were observed in transcriptional expression of TFRC, a cell membrane transferrin receptor related gene, and PMEPA1, a gene encoding a steroid‐dependent protein product. Expression of TFRC was down‐regulated while expression of PMEPA1 was up‐regulated by SULT1E1 transfection in CA‐HPV‐10 cells. The DNA microarray results were further confirmed by real‐time RT‐PCR analysis. These results indicated a regulatory role of SULT1E1 in expression of signal transduction‐related genes in CA‐HPV‐10 cells. Future studies will focus on the molecular mechanism underlying the observed effects of SULT1E1.