Premium
Intermittent hypoxia conditioning protects against mitochondrial damage induced by ethanol withdrawal in female rats
Author(s) -
Jung Marianna,
Downey Fred,
Simpkins James,
Wilson Andrew,
Confessore April,
Mallet Robert
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1175
We tested whether intermittent hypoxia conditioning (IHC) protects against adverse effects of ethanol withdrawal (EW) in female rats. Ovariectomized rats with or without 17β‐estradiol (E2) replacement received a control dextrin or an ethanol diet (6.5%, 5 wks). During the last 20‐diet‐days rats received IHC: repetitive (5–8/d), brief (5–10 min) periods of hypoxia (9.5–10% O 2 ). At 24 h of EW, rats were tested for EW signs, and protein oxidation and mitochondrial integrity were assessed by measuring carbonyls and absorbance at 540 nm, respectively (Table: ∗P<.05 vs dextrin; † P<.05 vs EW; ‡ P<.05 vs EW/E2). EW rats had significantly elevated EW signs, carbonyls, and evidence of impaired mitochondrial integrity (time to 50% absorbance, reflective of in vitro membrane rupture). IHC sharply reduced signs of EW and improved mitochondrial integrity; these variables were further improved by E2 co‐treatment. IHC may have therapeutic value in aiding EW.