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Short exposure to particulate matter from vehicle traffic promotes oxidative stress in lung of rats
Author(s) -
Rhoden Claudia Ramos,
Pereira Carlos Eurico Luz,
Heck Thiago Gomes,
Saldiva Paulo Hilário Nascimento
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1171-c
Subject(s) - oxidative stress , particulates , bronchoalveolar lavage , lung , toxicity , chemistry , ventilation (architecture) , zoology , medicine , toxicology , environmental chemistry , biology , organic chemistry , mechanical engineering , engineering
In vitro and in vivo studies have demonstrated chronic and acute toxicity of particulate matter (PM). However, in majority these studies have been performed using high doses of PM. The aim of this study was to verify whether ambient level of PM promotes oxidative stress, and inflammatory responses in lung of rat. Adults, male, Wistar rats were exposed to ambient particles (polluted) or filtered air (control) for periods of 6 or 20 hours in Porto Alegre city, Brazil. Exposure chambers were made of glass and one (control) received a Teflon filter in the inlet (PM<10μm), whereas the other received atmospheric air (polluted). After the exposure animals were euthanized to collect lung for oxidative stress (OE) evaluation and edema. Another set of animals followed the same protocol and submitted to bronchoalveolar lavage (BAL) 24h later to determine inflammatory parameters. Rats breathing polluted air for 20h showed increase of OE in lung tissue ([MDA] nmol/mg protein: polluted=0.226±0.02 X control=0.148±0.01 ‐ p = 0.040) and increase of total cells (X105) in BAL (polluted=2.61±0.51 X control= 5.01±0.81‐ p=0.041). There was not difference in theses outcomes in the group of rats breathing pollution for 6 h (total cells – polluted= 3.86±0.42 X control=3.02±0.37 p=0.464; OE ([MDA] nmol/mg protein: polluted=0.145±0.01 X control=0.142±0.01‐ p=0.950). The OE imposed by 20h exposure to polluted air was not associated with significant increases in the lung water content (p>0.05). This study shows that short‐term inhalation of low concentrations of PM in “real world” exposure promotes biological hazards. Supported by: FFFCMPA, CAPES, CNPq.