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Histamine modifies the expression of metalloproteinase in tissue of patients with cervix uterine cancer
Author(s) -
Ayala Elizabeth Álvarez,
Sánchez José Neri,
León Francisco Jurado,
Fragoso Lourdes Rodriguez,
ReyesEsparza Jorge
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1150-d
Subject(s) - histamine , proteases , matrix metalloproteinase , extracellular matrix , cancer research , cancer , protease , metalloproteinase , urokinase , pathology , biology , chemistry , medicine , endocrinology , enzyme , microbiology and biotechnology , biochemistry
Metalloproteinases and urokinase are involved in the process of degradation of basement membrane and extracellular matrix in tumoral tissue. Therefore this, they participate in invasion of tissues and metastases generation. Histamine is a chemical messenger that acts as a growth factor or chemo‐attracting. At present the role of histamine in cancer is not clear. The aim of this work was to evaluate the effect of histamine on expression of proteases on explants of patients with cervix uterine cancer. We used biopsies of 50 patients with cancer and 10 biopsies from normal tissue. Tissue was cultured with DMEM, transferring 10ƒÝg/ml and non essential amino acids. The tissue was treated with 100 ƒÝƒ½ histamine. The proteases expression was evaluated by a immunoblot using monoclonal antibodies against each protease. Our results showed that histamine induces the proteases expression in tissue from patients with and without cancer. Histamine changed the patron of proteases expression from MMP ‐1 and MMP‐3 to MMP‐2 and MMP‐9 in some samples of cancer tissue. On the other hand, histamine induced the expression of MMP‐1 (66%), MMP‐3 (75%), MMP‐7 (8%), MMP‐9 (41%) and MMP‐13 (8%), as well as urokinase (8%). This results showed that histamine is able to induce the protease expression in normal and malignant tissue and modifies the patron of proteases to one more invasive. Therefore, histamine should to play an important role in the process of invasion and metastasis.