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Coxsackievirus A9, unlike typical enteroviruses, does not shut off host cell protein synthesis
Author(s) -
de la Torre Alfred,
Handy Jean,
Beck Melinda A.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1148-c
Subject(s) - coxsackievirus , vero cell , virology , shut down , biology , cell culture , cell , lysis , enterovirus , protein biosynthesis , microbiology and biotechnology , virus , chemistry , biochemistry , genetics , computer science , operating system
Coxsackievirus A9 (CA9) is a human enterovirus of the Picornaviridae family, which are generally known to vigorously take over the protein synthesizing apparatus of the infected cell, and in the process, shut down the production of endogenous cellular proteins. Two strains of CA9 (including Griggs) were compared with one strain of Coxsackievirus B3 (CB3) in three different cell types: DBS‐FRhL‐2, Vero, and LLC‐MK2. All three viruses produced evident cytopathic effect within 12 hours in all three cell types. Cells were grown in 12‐well plates and exposed to [ 35 S] labeled methionine/cysteine amino acid mix for a pulse of 30 min at 1, 3, 5, 7, and 9 hours after infection. Cells were then lysed and proteins were separated by electrophoresis after adjusting samples for protein content. Electrophoresis gels were then dried and exposed to a phosphor imaging plate and read in a phosphorimager. As expected, host cell protein synthesis in CB3 infected cells was noticeably decreased by 5 hours post infection, and continued to diminish at 7 and 9 hours. However, neither of the CA9 strains demonstrated any host cell shut‐off. Both CA9 viruses as well as CB3 produced viral proteins by 5 hours post infection. We conclude that CA9 viruses do not behave as typical enteroviruses in that they do not shut down host cell protein synthesis. Supported by NIH grant R01EY12310