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A new epithelial mapping system use cytology prep to detect gallbladder adenocarcinoma on the operative room
Author(s) -
Alvarez Hector Andres,
Zarate Alejandro,
Castiblanco Adriana,
Madrid Patricio,
Aguayo Gloria,
Giannini Mary J,
de los Angeles Alvarez Maria,
Duarte Ignacio,
Pimentel Fernando,
Ibanez Luiz,
Diaz Alfonso,
Corvalan Alejandro
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1138-c
Subject(s) - adenocarcinoma , medicine , cytology , cholecystectomy , gallbladder , pathology , population , sampling (signal processing) , cancer , radiology , environmental health , filter (signal processing) , computer science , computer vision
Gallbladder Adenocarcinoma is one of the leading causes of cancer death in Chilean population. Epithelial scrapping cytology is a common procedure for exclusion of adenocarcinoma. We evaluated the use of cytological preps to determine whether information obtained is equivalent to that found with conventional gallbladder mapping techniques on frozen (FT) and formalin‐fixed, paraffin‐embedded (FFPE) preparations. 110 prospectively collected gallbladders, gallstone associated, were included. For each surgical specimen 6 different epithelial surfaces were cell scrapped, extended in a glass slide and methanol fixed (EASI prep). Paired FT and FFPE sampling were performed from each area evaluated. EASI preps follow standard H&E and 17 cytological variables were evaluated independently by two cytopathologists. 645 EASI preps were isolated from sample size and 4 patients with Adenocarcinoma were identified after histological follow‐up. Cytological features were matched to final diagnostic to see capability to detect cancer cells. In brief, three cytological features were considered diagnostic with a sensibility of 100% and Specificity range 71–99%. This fast, inexpensive and non‐depended skill technique is equivalent to conventional pathological approach and could help us to detect adenocarcinoma in patients undergoing cholecystectomy, especially with unapparent tumors.