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Nephropathy complications in the hyperglycaemic Psammomys obesus, an animal model for type 2 diabetes
Author(s) -
Bendayan Moise,
Ziv E.,
Londono I.,
Katalan S.,
Got G.,
Pizov G.,
Scherzer P
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1132
Subject(s) - medicine , endocrinology , diabetic nephropathy , glomerular basement membrane , diabetes mellitus , creatinine , excretion , type 2 diabetes , renal function , basement membrane , nephropathy , kidney , biology , pathology , glomerulonephritis
The Psammomys obesus lives on low energy diet in its natural desert habitat. However, in laboratory conditions under high energy diet, it exhibits metabolic symptoms of type 2 diabetes and is becoming an excellent animal model for type 2 diabetes. The present study has evaluated histo‐pathological and metabolic renal alterations occurring in this animal under short term hyperglycaemic conditions. Renal functions were characterized by measuring GFR, protein excretion, protein/creatinine ratio and by morpho‐immunocytochemical evaluations of the renal tissue. The hyperglycaemic animal presents a 54% increase in GFR after one month of hyperglycaemia and a decrease of 47% after 4 months of hyperglycaemia. Protein excretion was significantly increased (2.50±0.45 vs 0.50±0.02 mg/24h) and electron microscopy immunocytochemical quantitative evaluations revealed the passage of serum albumin across the glomerular wall (0.44±0.01 vs 0.35±0.07, p<0.005). Furthermore, although the thickness of the glomerular basement membrane did not increase significantly after 3 months of hyperglycaemia (164.75±9.90 vs 159.25±9.10 nm), focal accumulations of basement membrane material as well as splitting of the glomerular basement membrane were frequent. In addition, glycogen‐filled Armanni‐Ebstein clear cells were numerous in the distal tubules including the thick ascending limbs of hyperglycaemic animals. These renal complications which consist of physiological and histo‐pathological changes are very similar to the clinical profiles of diabetic patients. The Psamommys obesus appears as a quite valuable animal model for human type 2 diabetes.