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Early Disappearance of Smoth Muscle Cells in a Rat Model of Vein Graft Arterialization
Author(s) -
borin thaiz ferraz,
Miyakawa Ayumi Aurea,
Cardoso Leandro,
Krieger José Eduardo
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1128-b
Subject(s) - anatomy , smooth muscle , vein , medicine
We characterized the morphometric and cellular events associated to arterialization of rat jugular vein up to 90 days. The rat right carotid artery was connected to the right jugular vein. The arterialized jugular and contralateral vessels were harvested for morphometry, and analysis of cellular density and apoptosis by Hoeschst 33258 and TUNEL, respectively. Apoptosis were markedly increased on the 1 st day (fig. A) and α‐actin immunostain for smooth muscle cell (SMC) almost disappeared by the 3 rd day. At the 7 th day, cellular density (fig. B) and SMC proliferation gradually increased till the 90 th day. There was a gradual thickening of the medial layer and formation of neointima with deposition of SMC in the subendotelial layer from day 7 on. Initially the medial layer appeared disorganized, day 7 to 14, then by day 28 it became more organized and the presence of an intimal layer with SMCs was evident. The neointima layer increased gradually from day 7 on (area in mm 2 : 0: 13.17±4.5, 1d: 11.42±6.0, 3d: 64.85±47.1, 7d: 88.98±91.5, 14d: 193.12±93.8, 28d: 361.23±108.3, 90d: 639.0±249,6). The hystological events associated to arterialization of jugular vein in rats largely resemble to what happens with human vein grafts and we want to use this model to identify and validate molecular targets and interventions to improve the long term patency of vein grafts. A and B