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Prevention of LPS‐induced cyclooxygenase‐2 expression in human pulmonary epithelial cells by N‐acetylcysteine
Author(s) -
Choi Sang yong,
Yu Ji Hoon,
Kim Hyeyoung
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1107-d
Subject(s) - a549 cell , lipopolysaccharide , cyclooxygenase , reactive oxygen species , acetylcysteine , pharmacology , chemistry , cell culture , inflammation , antioxidant , microbiology and biotechnology , cell , immunology , biochemistry , medicine , biology , enzyme , genetics
Cyclooxygenase‐2 is suggested to play an important role in pulmonary inflammation response. N‐acetylcysteine (NAC) is an antioxidant with scavenging action against reactive oxygen species (ROS). This study aims to investigate whether lipopolysaccharide (LPS) induces COX‐2 expression through ROS generation and NAC inhibits LPS‐induced COX‐2 expression in human pulmonary epithelial cell line (A549). After LPS (5, 10ug/ml) was treated to A549 cell, ROS production was monitored using DCFDA assay. For inhibition of COX‐2 expression, LPS (0.5ug/ml) was treated with NAC (1mM) in A549 cells. COX‐2 expression was determined by Western blotting. The results show that LPS continuously produces ROS up to 1‐hour culture and induces COX‐2 expression in A549 cells time‐ and dose‐ dependently. LPS‐induced COX‐2 expression was inhibited by NAC in A549 cells. In conclusion, radical scavenging effect of NAC may inhibit LPS‐mediated COX‐2 expression in A549 cells. (This study was supported by Brain Korea 21 Project, Yonsei University College of Human Ecology)