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Hepatic Lipase ‐514C>T Polymorphism Modifies the Association between Dietary Fat Intake and Fasting Levels of HDL‐cholesterol in Individuals with Type 2 Diabetes
Author(s) -
FontaineBisson Benedicte,
Wolever Thomas MS,
ElSohemy Ahmed
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1105-b
Subject(s) - medicine , endocrinology , polyunsaturated fat , type 2 diabetes , cholesterol , population , genotype , blood lipids , apolipoprotein b , hepatic lipase , saturated fat , diabetes mellitus , biology , lipoprotein , genetics , gene , environmental health
Heterogeneity in circulating HDL‐cholesterol concentrations in response to dietary fat intake may be due, in part, to genetic variations in the hepatic lipase (HL) gene. A polymorphism in the HL gene (−514C>T) has been shown to modify the effect of dietary fat on circulating HDL‐cholesterol concentrations in some cohort studies, but the effects have not been consistent. We investigated the gene‐diet interaction in a population of Canadian individuals with type 2 diabetes. Subjects (n=109) recruited were type 2 diabetic men (n=53) and women (n=56) aged 42–75 years. Blood samples were collected to determine fasting plasma lipid levels and genotyping was performed by PCR‐RFLP. An analysis of covariance was performed for age, sex, BMI and alcohol intake. We found an interaction between the HL −514C>T polymorphism and intakes of total fat (p=0.005), saturated fat (p=0.02), and monounsaturated fat (p= 0.01), but not polyunsaturated fat (p= 0.10) on serum HDL‐cholesterol. Energy from total fat intake was inversely associated with serum HDL‐cholesterol for individuals with the T allele (regression coefficient β =−0.02±0.01 mmol/l /1% energy from fat; p=0.007), but not among those with the CC genotype (p=0.31) (p for interaction =0.005). All interactions were observed among non‐obese, but not obese individuals. In summary, the HL −514 C>T polymorphism modifies the association between dietary fat intake and serum HDL‐cholesterol in non‐obese individuals with type 2 diabetes. Supported by CIHR (MCT‐44205).