Premium
Individual and combined effects of docosahexaenoic acid, stearic acid, trans‐vaccenic acid, and c9,t11‐conjugated linoleic acid on hepatic gene expression
Author(s) -
Wang Long,
Zou Mi,
Koser Stephanie L.,
Story Jon A.,
Donkin Shawn S.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1102-c
Subject(s) - docosahexaenoic acid , conjugated linoleic acid , polyunsaturated fatty acid , linoleic acid , stearic acid , vaccenic acid , fatty acid synthase , chemistry , fatty acid , carnitine , biochemistry , metabolism , fatty acid desaturase , organic chemistry
Dietary long‐chain fatty acids (LCFA), polyunsaturated fatty acids (PUFA) and their combinations alter metabolism by modulating expression of fatty acid synthase (FAS), carnitine palmitoyltransferase‐1a (CPT‐1a), and acyl‐CoA oxidase (ACO). The effects of LCFA and PUFA on mRNA expression were determined in H4IIE rat hepatoma cells incubated with docosahexaenoic acid (DHA), c9, t11‐conjugated linoleic acid (CLA), trans ‐vaccenic acid (TVA), and stearic acid (SA) or their combinations. Treatments were: 200 μM of individual fatty acids DHA, CLA, SA, or TVA; Replacement of 100 μM DHA with either 100 μM CLA or 100 μM SA; 100 μM CLA replaced by 100 μM TVA (final fatty acid concentration of 200 μM); 200 μM of DHA or CLA supplemented with 200μM of CLA, SA, or TVA. When CLA replaced DHA, FAS mRNA decreased by 53.7% (P< 0.05). Replacement of DHA with SA resulted in a 74.9% decrease (P< .05) in FAS mRNA. When 200 μM CLA was supplemented with 200 μM DHA or with 200 μM TVA, FAS mRNA decreased (P< 0.05) by 62.4% and 48.6% respectively. When CLA was replaced with TVA, CTP‐1a mRNA tended (P= 0.05) to decrease by 47.5%. When CLA was supplemented with DHA there was a tendency (P< 0.10) for a 40.2% decrease in CPT‐1a mRNA. Fatty acid concentration and mixture had no effect on ACO mRNA. The data suggest that individual and combined LCFA/PUFA exert different potencies on expression of genes for fatty acid metabolism in liver.