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Effect of IGF‐1 restoration on phospholipid profile in skin tissues of weight controlled mice by exercise
Author(s) -
Jiang Yu,
Chen Jie,
Dong Lei,
Ouyang Ping,
Wang Weiqun
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1099-a
Subject(s) - lysophosphatidylcholine , medicine , endocrinology , chemistry , phospholipid , lipidomics , phosphatidylinositol , body weight , signal transduction , biochemistry , membrane , phosphatidylcholine
Studies in animal models demonstrated that weight control via physical activity had cancer preventive effect. We found the circulation level of IGF‐1 and IGF‐1 dependent signaling decreased in weight controlled mice by exercise. To further confirm the mechanistic role of IGF‐1, we restored IGF‐1 in SENCAR mice that were pair fed with the sedentary control and exercised by treadmill at 0.5 mph for 90 min daily, 5 days per week, for 10 weeks (PE). IGF‐1 was restored by i.p. injection at 10 μg/kg B.W. twice per week in the last two weeks. The results indicated that the body weight was significantly decreased in PE mice when compared to the control. Fat composition in IGF‐1‐injected mice was brought back to the levels of the control, but significantly higher than the PE mice. Total 338 phospholipids from 12 species in the skin tissues were detectable by lipidomics and the percentage of phosphatidylinositol 38:4 was significantly reduced in PE mice but increased in IGF‐1‐restored mice. Furthermore, IGF‐1 injection significantly enhanced some lysophosphatidylcholine species such as 20:3, 20:4, and 20:5 in PE group in comparison with the control. The study showed that some phospholipids that related to signaling pathway, cell proliferation, and/or cell death were selectively reduced by PE treatment and IGF‐1 restoration seemed to reverse, at least in part, the impact (supported by NIH R01CA106397).