Phospholipid profiling and related protein expression in skin tissues of body weight controlled mice

Our previous studies in a mouse skin cancer model via treadmill exercise or dietary calorie restriction (DCR) have suggested a significant reduction of body weight and body fat might prevent cancer by attenuating TPA‐induced PI3K signaling. Our recent lipidomics data showed a significant alteration of phospholipid profiles between exercised mice and sedentary control. Among of which, some ?‐3 fatty acyl combinations in phosphatidylcholine and phosphatidylethanolamine species increased significantly in exercise and pair‐fed mice when compared to the controls. Gene expression as measured by microarray data demonstrated a significant up‐regulation of genes related to elongation of very long fatty acids in DCR‐fed mice. Furthermore, proteomics analysis including over 600 protein spots in each 2D gel has been recently established in our lab for identification and quantification via matrix‐assisted laser desorption/ionization mass spectrometry. A differential expression of certain proteins such as apolipoprotein A1 and transgelin 2 was found to be significantly reduced in DCR mice. Consideration of the profiling changes in signaling phospholipids, lipid metabolism‐related gene and protein expression together may ultimately help us to understand the mechanisms by which weight control may protect against cancer (support by NIH CA106397 ).