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Effects of Anti‐inflammatory Quercetin and Kaempferol on Cell Growth and the Production of Angiogenic Factors in HT‐29 Human Colon Cancer Cells
Author(s) -
Lee EunJu,
Choi EunJin,
Choi ChoongIl,
Park JongSug,
Sung MiKyung
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1092
Subject(s) - kaempferol , quercetin , cell growth , secretion , angiogenesis , chemistry , cancer cell , pharmacology , cancer research , cell , cancer , medicine , biochemistry , antioxidant
Polyphenols have been reported to possess anti‐inflammatory activity, thereby act as chemopreventive agents. The objective of this study was to evaluate quercetin and keampferol as anti‐inflammatory agents, and to assess their effects on colon cancer cell growth and angiogenic factor production. Human colon cancer cells (HT‐29) were treated with 20 and 50 uM of quercetin or kaempferol for 24 to 72 hrs. The level of NO and PGE 2 in culture media were measured as inflammatory markers for inflammatory responses. Cell proliferation, MMP activity and VEGF secretion were measured. Results showed that quercetin and kaempferol at 20~50uM reduced NO production, while PGE 2 secretion was suppressed only by kaempferol. Both flavonoids inhibited cell proliferation in a dose‐dependent manner. No synergistic effect on cell growth in inhibition was observed in 1:1 combination treatment. Kaempferol at 25 uM significantly decreased MMP‐2 activity and VEGF production. These results suggest that quercetin and kaempferol possess cancer cell growth inhibitory effects. Keampferol exhibited superior anti‐inflammatory activity, thereby significantly reduce angiogenic factor production. This work was funded by the Rural Development Adminstration (BIOGREEN 21 PROGRAM, 20050501‐034‐831‐006‐02‐00).