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Antioxidant Effects of Pomegranate Husk (Granati pericarpium) in C57BL/6J Mice Fed High Fat and Cholesterol Diet
Author(s) -
Kim Mee Ree,
Kwon Oh Yun,
Sok Dae Eun
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1084-b
Objective: Pomegranate is a rich source of antioxidants such as polyphenolic(ellagic tannin, ellagic acid, punicalagin) and anthocyanis. The present study evaluated that the effects of pomegranate husk (Granati pericarpium) antioxidant biomarkers in mice fed high fat and cholesterol diet. Method: C57BL/6J mice were feed a American Institute of Nutrition AIN‐93G Diet(Dyets Inc., PA, USA) added 20% fat (corn oil & lard and) 1% cholesterol and 0.3% pomegranate peel EtOH extract for 4 weeks. The lipid profiles, lipid peroxidant value, antioxidant enzyme activity and DNA damage were evaluated. Results: The distribution of body fat deposition was significantly decreased in pomegranate husk groups. The activities of GPx, GST and SOD were significantly higher in liver or plasma of pomegranate husk group than those in control group. Also, pomegranate husk diet was decreased lipid peroxidation of liver and kidney. Commet assay (single cell gel assay) with alkaline electrophoresis showed that the DNA damage of plasma in pomegranate husk group was decreased, compared with that of control. Discussion: The present results show that diet added pomegranate husk exerts degenerative disease‐protective effects on oxidative DNA damage and lipid peroxidation possibly via a free radical levels in mice fed high fat and cholesterol diet.