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Protective potential of mistletoe (Viscum album coloratum) against oxidative stress and cellular premature senescence
Author(s) -
Kim BohKyung,
Park KunYoung,
Ban YoungEun,
Cho EunJu
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1084
Subject(s) - oxidative stress , viscum album , chemistry , nitric oxide , peroxynitrite , reactive oxygen species , viability assay , pyrogallol , microbiology and biotechnology , biochemistry , superoxide , cell , biology , traditional medicine , medicine , organic chemistry , enzyme
The protective activity from oxidative stress and antiaging potential of mistletoe ( Viscum album coloratum ) were investigated under in vitro and cellular system using LLC‐PK1 renal epithelial cell and WI‐38 human lung fibroblast cells. The water extract from the mistletoe (Korean product) showed nitric oxide (NO) and hydroxyl radical scavenging activity as concentration dependent‐manner. Moreover, under LLC‐PK1 cellular model, the mistletoe extract ameliorated oxidative stress induced by 2,2′‐azobis (2‐amidinopropane) dihydrochloride, the generator of peroxyl radical. In addition, the protective effect of mistletoe from NO under cellular oxidative stress was also studied. The LLC‐PK1 cells showed the decreases in cell viability by the treatment with generators of peroxynitrite, 3‐morpholinosydnonimine, and its precurors NO and superoxide anion produced by sodium nitroprusside and pyrogallol, respectively. However, the mistletoe led to the significant increases in the cell viability with dose‐dependence. Furthermore, the protective potential from oxidative stress‐induced aging was studied using WI‐38 cells. The WI‐38 cells exerted the characteristics of cellular premature senescence by the treatment of hydrogen peroxide, but mistletoe protected against stress‐induced cellular senescence. The present study suggests that mistletoe extract would have the protective activity from oxidative stress caused by free radicals and the promising anti‐aging potential against oxidative stress‐induced aging process.