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Anti‐inflammatory activities of the extracts of Compositae sp. for the prevention of adhesion between monocytes and human vein endothelial cell
Author(s) -
Lee JeHyuk,
Park HyunSun,
Lee Yong Soo,
Kim GunHee
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1083-b
Subject(s) - cell adhesion molecule , cell adhesion , soluble cell adhesion molecules , intercellular adhesion molecule , endothelial stem cell , adhesion , intercellular adhesion molecule 1 , human umbilical vein endothelial cell , inflammation , e selectin , immunology , chemistry , biology , microbiology and biotechnology , cell , biochemistry , in vitro , organic chemistry
In inflammatory responses, especially Rheumatoid arthritis, the adhesion between the monocytes and human vein endothelial cell (HUVEC) acts as one of the initial steps for inflammation response. When the human vein endothelial cells in bloodstream are exposed at the inflammation mediators, such as interleukin‐1 and tumor necrosis factor (TNF‐alpha), the cell adhesion molecules such as vascular cell adhesion molecule‐1 (VCAM‐1) and intracellular adhesion molecule‐1 (ICAM‐1), endothelial‐leukocyte adhesion moleculae‐1 (E‐selectin), and platelet endothelial cell adhesion molecule‐1 (PECAM‐1) are expressed for the attachment to monocytes. Compositae sp. is used for the activation of hepatocytes, the recovery of liver functions, and hepatitis in Korean traditional pharmaceuticals. We investigated the effects of Compositae sp. plants (Inula helenium, Gardenia jasminoides, safflower, Atractylodes japonica)‐extracts as anti‐inflammatory agents on inhibition of the adhesion between monocytes and HUVEC and the expression of CAM. Firstly several properties of biochemical functions for scavenging the reactive oxygen species were determined. And through cell proliferation test, we determined the concentration of extracts for the cell‐cell adhesion and the toxicity for human endothelial cell line. Through the cell‐cell adhesion assay, the extracts (25 ug/ml) in hexan layer of Inula helenium showed about 90 % inhibition between monocytes and HUVEC. Also the inhibition of adhesion was caused by down‐regulation of CAM protein expression in HUVEC. We expect that extracts of Compositae sp. plant may be used as the functional food materials and an alternative medicine to help for the Rheumatoid arthritis symptoms.