Premium
Evaluation of the potential efficacy of a natural rooster comb extract (Hyal‐Joint®) in maintaining joint integrity and function
Author(s) -
Torrent Anna,
Ruhí Ramon,
Ubia Anna,
Alcaraz Mª José,
Blanco F.J.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1082-c
Subject(s) - hyaluronic acid , catabolism , chemistry , endogeny , metalloproteinase , dermatan sulfate , biochemistry , matrix metalloproteinase , glycosaminoglycan , pharmacology , biology , enzyme , heparan sulfate , anatomy
Purpose: To evaluate the potential chondroprotective activity of a natural rooster comb extract (Hyal‐Joint®) containing mainly Hyaluronic Acid (HA) and Dermatan Sulfate (DS) at approximately 4:1, respectively, in comparison with Hyaluronic Acid (HA). Methods: Three in vitro assays were performed to determine the putative activity of Hyal‐Joint®. Its effect on the synthesis of endogenous AH was measured in synoviocytes from osteoarthritic patients (n=3) and was compared to HA from fermentation and HA from natural origin. The effect on catabolism (matrix metalloprotease activity and Interleukin‐6 production) was determined by using human osteoarthritic chondrocytes (n=4) stimulated with Interleukin‐1ß (IL‐1β) and treated with HA, DS and a mixture containing HA and DS in a 4:1 proportion. Results: In human synoviocytes, all 3 compounds exhibited a concentration‐dependent effect. Cultures treated with Hyal‐Joint® presented higher values of endogenous HA than the other two. This natural extract also showed inhibitory effects on catabolism, with a reduction of IL‐6 levels and a synergistic action between HA and DS when given together to decrease metalloprotease activity. Conclusions: The present study indicates that Dermatan Sulfate could potentiate the chondroprotective activity of Hyaluronan. Thus, Hyal‐Joint® may be a useful nutritional supplement for maintaining joint integrity and function.