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Growth‐Inhibitory Effect on Cancer Cells of the Extract from Crotalaria Sessiliflora L. and Identification of Anticancer Agents
Author(s) -
Kim Taesoo,
Kang Myung Hwa,
Park Hee Woon,
Park Chun Geon,
Seong Ha Jung,
Koh Sang Bum
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1081-c
In this study, with the extracts from different parts of Crotalaria sessiliflora L., we tested their cytotoxicity in and growth‐inhibitory effect on cancer cells through CytoScanTM‐TPM cytotoxicity and MTT assays and structurally identified unknown materials. The CytoScanTM‐TPM cytotoxicity assay found that the extracts from leaves, roots, and stems show concentration‐dependent significant differences (p<0.05, p<0.01) in the cell‐survival rate compared with the control group; and the MTT assay indicated that for the extract from leaves there is a statistically significant decrease (p<0.01) in the cell‐survival rate. Based on these findings, we collected and quantified a unknown peak, but only known as an effective component of Crotalaria sessiliflora L., using a Prep‐HPLC system and obtained a molecular weight of 325.35 from the quantified peak as determined by GC/MS. By means of 1H‐NMR and 13C‐NMR measurements, the peak was identified as monocrotaline with a chemical formula of C16H23NO6, where 23 protons and 16 carbons are included. When quantified by HPLC, the monocrotaline content was 9.18mg/100g in leaves, 9.30mg/100g in steams, 6.70mg/100g in roots, and 8.86mg/100g in seeds; As stated, the highest level was measured in stems. All these results suggest that further studies of Crotalaria sessiliflora L. will reveal its availability as both a natural antioxidant and an anticancer agent and its potential for prevention and treatment of cancer.