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Bifidobacterium lactis modulates local immune response and glucose uptake in the small intestine of pigs infected with parasitic nematode Ascaris suum
Author(s) -
SolanoAguilar Gloria Isabel,
SheaDonohue Terez,
Madden Kathleen,
Dawson Harry,
Jones Yolanda,
Restrepo Marta,
Urban Joseph
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1076-d
Subject(s) - ascaris suum , probiotic , immune system , biology , bifidobacterium , intestinal permeability , weaning , microbiology and biotechnology , ussing chamber , immunology , endocrinology , lactobacillus , food science , bacteria , helminths , secretion , genetics , fermentation
Hypothesis based testing of the effect of probiotic bacteria as a health promoting dietary intervention is dependent on careful measurement of immune and gut function. Bifidobacterium lactis (Bb12) (3.5 x 10 10 cfu) or a placebo containing vehicle was inoculated orally to pregnant sows during the last trimester of pregnancy and to their offspring from birth until weaning. Six weeks after weaning, piglets were infected with A. suum. After 21 days, stripped sections of jejunal mucosae were mounted in Ussing chambers to determine changes in permeability and glucose absorption. Tissue samples from jejunum, mesenteric lymph nodes and proximal colon were collected for gene expression analysis. Bb12 was detected in the highest concentrations in the colon of probiotic‐treated piglets. In the small intestine, probiotic treatment did not affect permeability but significantly attenuated the prototypical reduction in glucose absorption induced by A. suum . Probiotic‐treated animals also had a localized modulation of Th2 type signature molecules such as IL‐13 and IL‐9. These results suggest that a human‐derived probiotic species can affect local immune responses in pigs and improve intestinal absorption during a nematode infection.